Seminiferous tubule cannulation (STC): a new, sensitive technique for detecting gene transfer in developing sperm
نویسندگان
چکیده
منابع مشابه
Sperm-mediated gene transfer.
Since 1989, a new method for the production of transgenic animals has been available, namely sperm-mediated gene transfer (SMGT), based on the intrinsic ability of sperm cells to bind and internalise exogenous DNA molecules and to transfer them into the oocyte at fertilisation. We first described the SMGT procedure in a small animal model, with high efficiency reported in the mouse. In addition...
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Sperm mediated gene transfer (SMGT) could provide the opportunity to carry out transgenesis on a mass scale using spermatozoa as vectors for exogenous DNA. However, the efficiency of sperm-mediated DNA transfer is still questionable, and the mode of transmission to the egg has not yet been well understood. Our aim was to investigate the capacity of bovine spermatozoa to carry exogenous DNA and ...
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A transgenic animal as originally defined by Gordon and Ruddle (1981), is an organism carrying recombinant molecules that were intentionally introduced by human intervention. Classically this entailed the stable integration of the transgene within the host animal’s genome and its transmission to progeny through normal breeding programmes. Transgenic chicken holds great promise in basic biologic...
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ژورنال
عنوان ژورنال: Gene Therapy
سال: 2003
ISSN: 0969-7128
DOI: 10.1038/sj.gt.3301850